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AAT Bioquest

FAQ

Posted on May 2, 2023
Which technique is used to visualize DNA following a PCR reaction?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Proteinpolyacrylamide geldna ladder


Posted on May 2, 2023
How do DNA fragments move through the gel?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What is the significance of running an uncut DNA on electrophoresis gel?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
How does the size of the DNA fragment affect its movement or migration through the agarose gel during electrophoresis?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
When should I use 1kb DNA ladder?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleuspolyacrylamide geldna ladder


Posted on May 2, 2023
How is a DNA ladder visualized in gel electrophoresis?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide geldna ladder


Posted on May 2, 2023
How is a DNA ladder used in gel electrophoresis?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRpolyacrylamide geldna ladder


Posted on May 2, 2023
What are the limitations of post-electrophoresis staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What are the advantages of post-electrophoresis staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsProteinnucleic acidpolyacrylamide gel


Posted on May 2, 2023
What are the limitations of in-gel staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What should be considered when I do in-gel staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What are the advantages of in-gel staining?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gelprotein


Posted on April 26, 2023
What are the most common buffers used for agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 26, 2023
When should I use a TBE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 26, 2023
When should I use a TAE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on September 19, 2022
What is SDS-PAGE?
Chemical ReagentsAgarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)detergentpolyacrylamide gel


Posted on September 19, 2022
Why is SDS used in protein gel electrophoresis?
Agarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)Western Blotdetergentgene sequencingpolyacrylamide gel


Posted on June 1, 2020
How does a stacking gel work?
Antibodies and ProteomicsAgarose gel electrophoresisGel Electrophoresispolyacrylamide gel


Posted on June 1, 2020
What is the difference between stacking gel and resolving gel in SDS-PAGE?
Agarose gel electrophoresisGel Electrophoresispolyacrylamide gel


Posted on June 1, 2020
Why aren’t SDS-PAGE gels horizontal?
Agarose gel electrophoresisDNA and RNA QuantitationDNA and RNAGel Electrophoresis


Posted on June 1, 2020
What is gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA and RNAGel Electrophoresispolyacrylamide gel


Posted on May 18, 2020
What does EDTA do in agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisChelatorEDTAGel Electrophoresisbuffer


Posted on April 24, 2020
What is an appropriate incubation time for primary antibody in western blot analysis?
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingGel ElectrophoresisWestern Blotpolyacrylamide gel


Posted on April 24, 2020
What is the difference between western blotting and far-western blotting?
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingEnzymesGel ElectrophoresisProteinagarose gelpolyacrylamide gel


Posted on April 24, 2020
What is the major difference between Northern and Southern blotting?
Antibodies and ProteomicsAgarose gel electrophoresisDNA and RNAEnzymesGel ElectrophoresisNucleic AcidsProteinWestern Blotpolyacrylamide gel


Posted on April 17, 2020
What is the difference between uses of Agarose gel electrophoresis and Polyacrylamide gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)polyacrylamide gelMacromoleculeNucleic AcidsPhysiological Probes


Posted on February 4, 2020
What methods are currently used to quantify DNA?
DNA quantificationAbsorbanceFluorescenceAgarose gel electrophoresis